It is irrational to sacrifice large animals like cat, dog for requirement of one or two tissues. Pharmaceutical Undergraduate curriculum in India continues to follow an archaic syllabus set up decades ago, which has not kept pace with the technological progress. We can use isolated preparations obtained from cock, as this can be abundantly available in nearby slaughter house (almost free of cost). In the current study, Dose response curve (DRC) to acetylcholine (ACh) was obtained using isolated cock colon __ampersandsignamp; concentration of the unknown was found by interpolation method. Same study procedure repeated for isolated cock tracheal chain using Histamine as agonist. Results obtained with isolated cock colon __ampersandsignamp; tracheal chain preparation was promising and satisfactory. Cock colon can be used for studying potentiating effect of ACh by neostigmine, comparison of activities of different cholinergic agonists, three point/four point bioassay, determination of pA2 value. Cock tracheal chain can be used for bioassay of histamine, broncho-constriction and broncho- dilating activities. There is need to carry further research on isolated cock preparations to place them permanently under experimental in-vitro pharmacology.