Background: Hypercholesterolemia is associated with to coronary heart disease (CHD) which is the principal cause of morbidity and mortality worldwide. Over the last few decades the reputation of herbal remedies has increased globally due to its therapeutic efficacy and safety. Aim: This study was carried out to investigate the hepatoprotective potential of locally grown Hibiscus rosa sinensis flower extracts in diet induced hypercholesterolaemic rat hepatocytes. Methodology: Male Wistar rats (180-230gm) were divided into seven groups of six animals each (n=6). The first group served as the control. All the animals were induced hypercholesterolemia by feeding pure cholesterol and cholic acid orally mixing with coconut oil with 2:1 ratio for 4 weeks. Three groups were given HRS flower extracts orally, at doses of 80mg / kg, 160mg / kg and 240 mg / kg body weight once a day for 5 days (acute) and another three groups were given same doses of HRS flower extracts for 30 days (chronic). At the end of treatment duration all animals were sacrificed by cervical dislocation. Laparotomy was performed. Blood was collected by cardiac puncture and allowed to clot. Serum was separated for the estimation of lipid profile, liver enzymes, total protein, albumin levels and plasma were used to estimate the MDA levels. Atherogenic Index (AI) was calculated. Results: There was increase in body weight in cholesterol fed experimental animals which was reversed with HRS fed groups. There was a dose dependent increase in serum hepatic marker enzymes and total protein levels significantly (p__ampersandsigngt;0.001) in the cholesterol fed groups and reversed with HRS flower extract fed acute (p__ampersandsigngt;0.005) and chronic (p__ampersandsigngt;0.001) groups. Increase in blood MDA level were seen in hypercholesterolaemic groups and significantly reduced (p__ampersandsigngt;0.05) in HRS flower extract treated animals. Conclusion: The present study suggests that HRS flower extract could play a hepatoprotective role against hypercholesterolemia through the regulation of cholesterol levels and inhibition of lipid peroxidation.