Background of study: The efficacy of methanolic and petroleum ether fractions of Avocado pear (Persea americana Mill) seeds in diet-induced hyperlipidemic rats was studied. Aim: To determine hypolipidemic and antioxidant effect of petroleum ether and methanolic fraction of seeds of Persea americana on diet-induced hyperlipidemic rats. Methodology: Eleven groups of five rats each were employed which included normal control, the high fat diet (HFD) control group, HFD + 0.2mg/kgbw atorvastatin, and groups treated with different doses of methanolic or petroleum ether fractions for four weeks. Result: Quantitative phytochemical analysis revealed significantly (p0.05) differences between the normal and HFD control groups, while there was significant (p__ampersandsignlt;0.05) decrease in alkaline phosphatase level. Conclusion: The serum urea level decreased (p__ampersandsignlt;0.05) significantly in the treatment groups. The petroleum ether and methanolic fractions of Persea americana seeds have been shown to have hypolipidemic and hypocholesterolemic potentials as well as in vivo antioxidant activity in diet-induced hyperlipidemic rats.