Aim: This study aims to formulate and evaluate herbal gel preparations incorporating various extracts (aqueous, methanol, ethyl acetate, and petroleum ether) of Bougainvillea glabra to explore their potential as alternative treatments for acne, addressing the increasing resistance associated with conventional antimicrobial agents. Methodology: The herbal gel formulations (F1-F4) were prepared using Bougainvillea glabra extracts and subjected to a comprehensive evaluation, including physical characteristics, washability, skin irritation potential, spreadability, pH, viscosity, extrudability, swelling index, and accelerated stability, using standard protocols. Antibacterial efficacy was assessed by determining the minimum inhibitory concentration (MIC) against Escherichia coli and Bacillus subtilis, and the results were compared with a marketed herbal product and the standard drug Clindamycin. Results: Among the formulations, F2 exhibited optimal properties across the evaluated parameters. The extraction yield of Bougainvillea glabra was 11.21%. Antibacterial testing revealed that the aqueous extract exhibited minimal activity, while the methanol and ethyl acetate extracts showed moderate activity. The petroleum ether extract demonstrated the highest antibacterial efficacy among the extracts but remained less effective than Clindamycin. Formulations (F1-F4) mirrored the trends observed with the extracts but showed reduced antibacterial activity compared to the marketed herbal product. Conclusion: The herbal gel formulations containing Bougainvillea glabra extracts offer a potential alternative for acne treatment, with F2 showing the most promising results. While the petroleum ether extract demonstrated notable antibacterial activity, further optimization and in vivo studies are warranted to enhance their therapeutic potential and compare their efficacy with established products.