Aim: Methicillin resistant Staphylococcus aureus (MRSA) in hospital is troublesome. Healthcare workers (HCWs) can act as reservoir and may result in its spread to susceptible patients. The present study estimates the prevalence of staphylococcal and MRSA carriages among HCWs as well as the susceptibility pattern of these strains against other selected antibiotics. Methodology: A total of 94 health care personnel from surgery wards and operation theatres were screened for MRSA carriage by taking anterior nasal swab. MeReSa chromogenic agar was used as a screening method. Minimum inhibitory concentration (MIC) determination by Agar dilution technique was used as a confirmatory method. Antibiotic susceptibility testing (AST) was done for the selected antibiotics. Results: Samples of 94 HCWs were screened. S.aureus carriage was found out to be 70 % (66/94). MRSA carriage was found to be 27 % (25/94) by screening MeReSa chromogenic agar (bluish green colonies), whereas it was 19 % (18/94) by oxacillin Agar dilution MIC method. 11% of MRSA strains were intermediate sensitive (IS) to vancomycin. All the strains were sensitive to linezolide, tigecycline and vancomycin. Conclusions: Significant prevalence of nasal carriage of MRSA and S. aureus among HCWs was found. Chromogenic agar can be used as a screening method of MRSA carriage and MIC by agar dilution can be used as a confirmatory method. Multi-drug resistance was prevalent in both MRSA and MSSA strains which is a worrisome fact.