Background: Maintaining a normal level of Vitamin D is crucial in order to have normal skeletal and extra-skeletal health. Vivitamin D deficiency is considered a common problem not only in the United Arab Emirates but also worldwide. To the best of our knowledge, there is limited data available in the United Arab Emirates regarding vitamin D deficiency among infants. Aim: To measure and correlate the knowledge, attitude and practice of mothers of six months old infants in Abu Dhabi Island, United Arab Emirates, regarding their infants__ampersandsignrsquo; need for vitamin D supplementation. Methods: A predefined questionnaire was submitted to 245 mothers of 6 months old infants in all governmental ambulatory health care centers in Abu Dhabi Island from 1st October 2013 to 23rd January 2014. The questionnaire measured the knowledge, attitude and practice of mothers of six months old infants in Abu Dhabi Island regarding their infants__ampersandsignrsquo; need for vitamin D supplementation. Results: The results in this study revealed poor knowledge, attitude and practice among mothers regarding vitamin D supplement for their infants. Conclusion: All health care professionals are encouraged to educate mothers effectively in order to increase mothers__ampersandsignrsquo; compliance to vitamin D supplementation according to the practiced guide.