Background: Secondary alveolar bone grafting of the cleft alveolar ridge in the mixed dentition is a well-established treatment for patients with cleft lip and palate (CLP). Recently tissue engineering has become available as a regenerative treatment for bone defects; however little has been reported on application of tissue engineering for regeneration of cleft defect tissues. Mesmesenchymal derived stem cells were applied to different kinds of bone substitute in attempt to overcome the drawbacks of bone grafting. Materials and methods: Eight patients with alveolar cleft were included in this study; the cleft repair was done using bone marrow derived stem cell loaded on NanoBone__ampersandsignreg; scaffold. All patients were followed up clinically and radiographically for 6 months postoperatively. Results: The successful healing was observed without oronasal fistula. In all patients the closure of the alveolar arch and stabilization of the teeth adjacent to the cleft were achieved. The radio-opacity in serial computed tomography slicing the middle level of the alveolar cleft in the grafted region increased gradually over the time. Conclusion: Tissue engineered bone from mesenchymal stem cells may provide an acceptable alternative especially in the case of limited autograft availability or donor site morbidity