Introduction: Aim of the present study is to identify bacteria causing community acquired acute uncomplicated cystitis in adult women and detection of local antibiotic resistance pattern of isolates . Materials and Methods: The present prospective study was conducted at a tertiary care hospital and post graduate teaching institute. Total 149 patients of acute uncomplicated cystitis who had significantly positive bacterial culture for single bacterium species were selected for the study. Eight antibiotics were tested by modified Kirby Bauer disc diffusion method. Results: The uropathogens identified were Escherichia coli (78.53%), Klebsiella pneumoniae (4.70%), Proteus mirabilis (2.01%), Staphylococcus saprophyticus (12.75%) and Enterococcus fecalis (2.01%). Most isolates showed resistance against cotrimoxazole (trimethoprim plus sulfamethoxazole) and coamoxiclav (amoxicillin plus clavunalate). They were susceptible to fluoroquinolones, third generation cephalosporin (cefpodoxime) and nitrofurantoin. Conclusions: The most effective antibiotics in the present study for acute uncomplicated cystitis were levofloxacin and cefpodioxide