__ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; Aim: Infections due to gram negative bacteria (GNB) mostly affect debilitated patients in intensive care units (ICUs). They are difficult to be controlled and treated due to its prolonged environmental survival and its ability to produce resistance to multiple antimicrobial agents. The present study was carried out to ascertain the prevalence of carbapenem resistance and antibiotic resistance profiles of GNB isolates from ICU. Methodology: A total of 270 samples from patients of ICU __ampersandsignamp; NICU (Blood-194, CSF-21, Vitreous/ Aqueous fluid-20, Pleural/ Peritoneal fluid-35) were included in the study. BACTEC bottles and BACTEC__ampersandsigntrade; 9050 automated culture system was used. Positive GNB cultures were processed for identification and AST with Vitek-2. Results: Common isolates were Escherichia coli, Klebsiella pneumoniae, Pseudomomas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae and others were Sphingomonas paucimobilis, Salmonella paratyphi A and Serratia spp. Carbapenem resistance was 41% overall. Resistance to other broad spectrum antibiotics like amikacin, cefepime and colistin was also high. Tigecycline was the only available option with least amount of resistance except, Pseudomonas spp. which is a worrying fact. Conclusions: Direct identification and AST with Vitek-2 gave rapid and reliable results which leads to a significant reduction of patient__ampersandsignrsquo;s morbidity, mortality and medical care cost. Carbapenem resistance was significantly high which was also seen with other broad spectrum antibiotics like amikacin, cefepime and colistin. Tigecycline was the only available option which is a worrying fact. Considerable efforts will be required to maintain the effectiveness of higher antibiotics by rational use __ampersandsignamp; antibiotic stewardship.__ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp;