Background: Candida respiratory infection is one of the health challenges caused by Candida species, but it is not usually investigated. This study was carried out to characterize the isolates from the subjects and also determine the antifungal susceptibility profile of the various isolates. Methods: Expectorate sputum samples were received from the subjects early in the morning for two days. All the subjects were attending ART clinics and DOTS center for evaluation and chemotherapy. Samples were subjected to microscopy and culture. Identification was done by standard mycological methods. The MICs for a cross section of the isolates were determined by semi solid agar antifungal susceptibility (SAAS) testing method against fluconazole, amphotericin B, itraconazole, ketoconazole. Results: The prevalence rate of Candida infections was 40(14.7%). Isolates were identified after consecutive isolation from the second sputum sample. Candida albicans (80%) was the most prevalent isolate while Candida guilliermondii 1 (2.5%) was the least prevalent. A lower mean CD4 counts (133.47__ampersandsignplusmn;96.51 cells/__ampersandsignmicro;l) was reported for subjects with Candida infections compared to those without Candida infections (378.85__ampersandsignplusmn;254.56 cells/__ampersandsignmicro;l). There was a statistically significant difference in the CD4 counts of subjects with Candida infections and those without the infection (t=6.01, P amphotericin B__ampersandsigngt;itraconazole__ampersandsigngt;ketoconazole. Conclusion: This study has revealed respiratory candida infections as a mycoses burden among HIV positive patients in Calabar. It also showed that SAAS method is useful in determining antifungal susceptibility of Candida isolates with results available within 48 hours of fungal isolation. The method is affordable and useful in a resource poor setting like ours.