Radiance Research AcademyInternational Journal of Current Research and Review2231-21960975-5241EnglishN2024November14Pharmaceutical SciencesAssessment of Antioxidant Activity of Methanolic Extract of Momordica Charantia L. Seeds English0105Sonali DeokateEnglish Vinod PawarEnglish Madhuri HoleEnglishSonali Deokate, Anekant Education Society’s College of Pharmacy (Religious Minority Institute), Tandulwadi Road, T.C. College Campus, Baramati, 413102, Maharashtra, India.https://doi.org/10.31782/IJMPS.2024.141101Aim: The present investigation was aimed to evaluate the antioxidant activity of methanolic extract of Momordica charantia using albino rats. Material and Methods: Anti-oxidant activity of M. charantia evaluated by using superoxide anion scavenging activity, reducing power, flavonoids content, hydrogen peroxide radical scavenging assay method were adopted for screening the antioxidant activity. Ranitidine was used as standard then comparison between standard and test was carried out. Results: Due to the presence of saponins and flavonoids in M. charantia increases the antioxidant activity. The methanolic extract of M. charantia showed significant activity (p < 0.05). Conclusion: The result indicates that the action of methanolic extract of M. charantia shows anti- oxidant activity. EnglishAnti-oxidant, Momordica charantia, Methanolic, Extract, Albino rats, Activityhttp://ijcrr.com/abstract.php?article_id=263http://ijcrr.com/article_html.php?did=2631. Mosha T, Gaga H, Laswai H, Mtebe K. Effect of blanching on the content of antinutritional factor in selected vegetables. Plant Foods Hum Nutr. 1995; 47(4): 361-367. 2. Zhishen J, Mengcheng T. The determination of flavonoids content in mulberry and their scavenging effect on superoxide radicals food chem. 1999; 64: 555-559. 3. Wamer D, Sheng H. Oxidants, antioxidants and the ischemic brain. J Exp Bio. 2004; 18: 3221-3231. 4. Sies H. Oxidants stress; oxidants and antioxidants. Exp Physiol 82. 1997; 2: 291-295. 5. D Satish Kumar, K. Vamshi Shrathnath, P. Yogeshwaran, A.Harani, K. Sudhakar. A Medicinal Potency of momordica charantia. Int J Pharma Sci Review and Research. ISSN 0976- 044X, 2010. 6. Shetty A.K, Kumar G.S, Sambaiah K, Salimath P.V. Effect of bitter Gourd (Momordica Charantia) on Glycemic Status in Streptomycin Induced Diabetis Mellitus Plant Foods for Human Nutrition. 2005; 60: 109-112. 7. Henrique D.M. Coutinho, Jose G.M. Costa, Vivyanne S. Falcao- Silva. Effect of Momordica Charantia L. in the resistance to aminoglycosides in methicilin-resistance Staphylococcus aureus. 2010. 8. Chi-I Chang, hsin-I Tseng, Yun-Wen Liao, Chia- Hung Yen. In Vivo and In Vitro studies to identify the hypoglycemic constituents of momordica charantia wild variant WB24. Food Chemistry. 2010; 125: 521-528. 9. Balaraman A.K, Singh J, Dash S, Maity T.P. Antihyperglycemic and Hypolipidemic effect of Melothria Maderaspatana and Coccinia indica in Strptozotocin induced diabetes in rats. Saudi Pharma J. 2010; 18:173-178. 10. Karlsson J. Introduction to Nutraology and Radical Formation in Antioxidants and Exercise. Illinois: Human Kinetic Press. 1997: 1- 143. 11. Nagulendran K, Velavan S, Mahesh R and Begum H. In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes, 2007; 4: 440-449. 12. Khandelwal KR. Practical Pharmacognosy, Techniques and Experiments. Nirali Prakashan.2006; 16:149-156. 13. Ilhams Gulcin, Munir Oktay, Irfan K.V, Ali A. Determination of antioxidant activity of lichen Cetraria Islandica L. Ach. J. Ethanopharmocol. 2002; 79: 325-329. 14. Chakrabarti S, Naik A.S, Gali R.R. Phenylhydrazine mediated degradation of bovine serum albumin and membrane proteins of human erythrocytes. Bioch Biophy Acta.1990; 1028: 89-94. 15. Haber F and Weiss. Uber die Katalyse des Hydroperoxydase. Naturwissenschaften. 1995; 49: 345. 16. Bromme H.J, Morke W, Peschke E. Transformation of barbituric acid into alloxan by hydroxyl radicals: interaction with melatonin and with other hydroxyl radical scavengers. J Pineal Res. 2002;  33(4):239-247. 17. Juan C, Jordi F, Maria I.G. Mineralization of aromatics in water by sunlight- Assisted Electro- Fenton Technology in a Pilot Reactor. Environ. Sci. Technol. 2005; 39(6):1843-1847. 18. Jamuna S, Panulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extract of Hypochaeris radicata L. J App Pharma Sci. 2012;07:149-154.